General Preferences: Lens

Use this tab to set options to compensate for differences between lenses.

Corrections

Available if Parcentric/Parfocal calibration has been performed.

Corrections are automatically applied whenever you change lenses.

Enable Parcentric: Microlucida automatically compensates for collimation differences between lenses.
Enable Parfocal: Microlucida automatically compensates for differences in focal planes between lenses.
Center View: Once a tracing has been aligned for a different lens, Microlucida moves the stage so that the center view for the new lens is at the same location as the center view for the previous lens.

Autofocus

Virtual Tissue is required for this option.

You can define the Z range for each lens to be used with Virtual Tissue. The higher power the objective, the smaller the Z range and the finer the step size.

Z range = steps to move above and below Z * step size
Step size: Minimum amount to move in Z.

Example

Steps to move above and below Z = 10
Step size = 2 microns

Then Autofocus is applied 20 (10*2) microns above and below Z.

If Autofocus is enabled in the lens preferences, you can also set the autofocus at the time of a Virtual Tissue acquisition: Click the Set Autofocus Range button in the Virtual Tissue Acquire window (in Acquisition Settings, see Settings>Focus Adjustments).

Microlucida 3 | MBF Bioscience Support Center | Downloads